Tunable Lattice-Form Mach-Zehnder Interferometer For Arbitrary Binary Code Generation At 40 Ghz

We use the direct temporal domain approach to design spectrally periodic optical filters for pulse repetition rate multiplication (PRRM) with envelope shaping. In particular, we demonstrate a tunable lattice-form Mach-Zehnder interferometer using Silica-based planar lightwave circuit (PLC) for arbitrary 4-bit binary amplitude code generation at 40 GHz and to increase the repetition rate of a 10 GHz input pulse train to 20 GHz or 40 GHz. In addition to PRRM and envelope shaping, the device also has the capability of arbitrary phase coding. © 2010 IEEE

Tunable Lattice-Form Mach-Zehnder Interferometer For Arbitrary Binary Code Generation At 40 Ghz

We use the direct temporal domain approach to design spectrally periodic optical filters for pulse repetition rate multiplication (PRRM) with envelope shaping. In particular, we demonstrate a tunable lattice-form MachZehnder interferometer using Silica-based planar lightwave circuit (PLC) for arbitrary 4-bit binary amplitude code generation at 40 GHz and to increase the repetition rate of a 10 GHz input pulse train to 20 GHz or 40 GHz. In addition to PRRM and envelope shaping, the device also has the capability of arbitrary phase coding. © 2006 IEEE

Vertically constrained CO_2 retrievals from TCCON measurements

Partial column-averaged carbon dioxide (CO_2) mixing ratio in three tropospheric layers has been retrieved from Total Carbon Column Observing Network (TCCON) spectra in the 1.6 μm CO_2 absorption band. Information analysis suggests that a measurement with ∼60 absorption lines provides three or more pieces of independent information, depending on the signal-to-noise ratio and solar zenith angle. This has been confirmed by retrievals based on synthetic data. Realistic retrievals for both total and partial column-averaged CO_2 over Park Falls, Wisconsin on July 12, 15, and August 14, 2004, agree with aircraft measurements. Furthermore, the retrieved total column averages are always underestimated by less than 1%. The results above provide a basis for CO_2 profile retrievals using ground-based observations in the near-infrared region

MHC Class II Expression Influences the Composition and Distribution of Immune Cells in the Metastatic Colorectal Cancer Microenvironment

Despite advances in therapy over the past decades, metastatic colorectal cancer (mCRC) remains a highly morbid disease. While the impact of MHC-I on immune infiltration in mCRC has been well studied, data on the consequences of MHC-II loss are lacking. Multiplex fluorescent immunohistochemistry (mfIHC) was performed on 149 patients undergoing curative intent resection for mCRC and stratified into high and low human leukocyte antigen isotype DR (HLA-DR) expressing tumors. Intratumoral HLA-DR expression was found in stromal bands, and its expression level was associated with different infiltrating immune cell makeup and distribution. Low HLA-DR expression was associated with increased intercellular distances and decreased population mixing of T helper cells and antigen-presenting cells (APC), suggestive of decreased interactions. This was associated with less co-localization of tumor cells and cytotoxic T lymphocytes (CTLs), which tended to be in a less activated state as determined by Ki67 and granzyme B expression. These findings suggest that low HLA-DR in the tumor microenvironment of mCRC may reflect a state of poor helper T-cell interactions with APCs and CTL-mediated anti-tumor activity. Efforts to restore/enhance MHC-II presentation may be a useful strategy to enhance checkpoint inhibition therapy in the future

Targeting Tumor Associated Phosphatidylserine with New Zinc Dipicolylamine-Based Drug Conjugates

A series of zinc­(II) dipicolylamine (ZnDPA)-based drug conjugates have been synthesized to probe the potential of phosphatidylserine (PS) as a new antigen for small molecule drug conjugate (SMDC) development. Using <i>in vitro</i> cytotoxicity and plasma stability studies, PS-binding assay, <i>in vivo</i> pharmacokinetic studies, and maximum tolerated dose profiles, we provided a roadmap and the key parameters required for the development of the ZnDPA based drug conjugate. In particular, conjugate <b>24</b> induced tumor regression in the COLO 205 xenograft model and exhibited a more potent antitumor effect with a 70% reduction of cytotoxic payload compared to that of the marketed irinotecan when dosed at the same regimen. In addition to the validation of PS as an effective pharmacodelivery target for SMDC, our work also provided the foundation that, if applicable, a variety of therapeutic agents could be conjugated in the same manner to treat other PS-associated diseases